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HPLC (High Performance Liquid Chromatography) Analysis
of the Kavalactones in Kava Extracts
Alden Botanica is pleased to offer HPLC Services for the analysis of your Kava. 
For pricing please contact us by e-mail.
Results of our analysis of Ethanol Extracts of Kava Root from Hawaii, Vanuatu, & Fiji, and a Supercritical Carbon Dioxide Extract by Reverse-Phase HPLC

Chemical Structure of the Kavalactones
Dr. John Pierce, President of Alden Botanica, using the HPLC to analyze a Kava extract A close-up of the HPLC equipment used to measure the kavalactones in our Kava
Dr. John Pierce using the HPLC equipment Close-up of the HPLC equipment


Chemical Structure of the Kavalactones

HPLC Chromatogram of an Ethanol Extract of Kava Fiji
HPLC Chromatogram of an Ethanol Extract of Kava Fiji Kavalactone
Distribution (%)
Methysticin         

24.41
Dihydromethysticin

14.97
Kavain 22.04
Dihydrokavain 22.64
Yangonin

10.21
Dehydrokavain

6.10

Chemical Structure of the Kavalactones

HPLC Chromatogram of an Ethanol Extract of Kava Hawaii
HPLC Chromatogram of an Ethanol Extract of Kava Hawaii Kavalactone
Distribution (%)
Methysticin         

17.14
Dihydromethysticin

13.39
Kavain 22.15
Dihydrokavain 29.48
Yangonin

10.29
Dehydrokavain

7.54

Chemical Structure of the Kavalactones

HPLC Chromatogram of an Ethanol Extract of Kava Vanuatu Lateral Roots
HPLC of Ethanol Extract of Kava Vanuatu Lateral Roots Kavalactone
Distribution (%)
Methysticin         

16.26
Dihydromethysticin

8.79
Kavain 29.12
Dihydrokavain 21.97
Yangonin

13.88
Dehydrokavain

9.98

Chemical Structure of the Kavalactones

HPLC Chromatogram of a Supercritical Carbon Dioxide Extract of Kava Vanuatu
HPLC of Supercritical CO2 Extract of Kava Vanuatu Kavalactone
Distribution (%)
Methysticin         

19.22
Dihydromethysticin

13.83
Kavain 23.10
Dihydrokavain 28.30
Yangonin

8.21
Dehydrokavain

7.29

 

 

 

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Revised: June 04, 2003.